Inventors at Georgia Tech have created a microfluidic device to electrically profile cell surface expression. The device combines differential sorting under various force fields and can be applied to sorting mechanisms, such as sorting by physical properties, dielectrophoresis, acoustics, and electro-osmosis. The inventors also developed a high dynamic range (HDR) technique to increase the device’s system resolution without adjusting the device’s design. They did this by manipulating the flow conditions in the device, producing a rage of operational conditions. This operational variation changes the effective work done by the magnetic force, and each flow condition provides unique information about the sample. Collectively analyzing data from the various flow rates results in improved operational resolution.
- Highly portable
- Easy integration
- Pre-sorted sample recovery
- High Dynamic Range technique-improved operation resolution
- Disease monitoring
- Biomedical Instruments
- Research Equipment
Cell surface antigens regulate biochemical interactions of cells, so measuring their expression levels is important in fields such as immunophenotyping, clinical diagnosis and prognosis, and biomedical research. Fluorescence-based flow cytometers are instruments for surface expression analysis that are bulky, expensive, and can only be operated by trained personnel.